1X Ni+ Binding buffer
     - 20mM NaPO4, 500mM NaCl pH7.4~7.8

     - add 500mM Imidazole for elution

 

4X SDS loading buffer
     - 200mM Tris-HCl (pH 6.8)
     - 8% SDS
     - 0.4% bromophenol blue
     - 40% Glycerol
     - (Optional) 400mM DTT

 

 

10X TBS in 1L
    - 1.5M NaCl (~87.66g)
    - 30mM KCl (~2g)
    - 250mM Tris

    - 0.5 phenol red (optional)

    - pH 7.8

10X PBS in 1L

   - 25.6 g Na2HPO4·7H2O

   - 80 g NaCl

   - 2 g KCl

   - 2 g KH2PO4
     - Bring to 1 liter with H2O.

 

 

10X TE buffer

    - 100mM Tris (pH 7.5)
    - 10mM EDTA
    - Sterilized

 

Superbroth
    - Tryptone, 32g

    - Yeast extract, 20g
    - NaCl, 5g

    - NaOH (1N), 5ml
    - ddH2O, 950ml

 

6X DNA loading buffer 
    - 30% Glycerol
    - 0.25% bromophenol blue
    - 0.25% xylene cyanol FF

    - Save 4C

  

10X Tris/Glycine for western blot
    - 250mM Tris
    - 1.92M Glycine
    - pH8.3 (don’t add NaOH or HCl to adjust pH)

 

Alkaline Lysis Solution II (P2 buffer of Qiagen Miniprep kit)
   - 0.2N NaOH (Freshly diluted from a 10N Stock)
   - 1% SDS

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